Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, "Conservation, Development and Utilization of South American Oil Vine (Plukenetia volubilis Linneo)", "Physical Development and Pedigree Biogeography of the Himalayan Endemic Plant Roscoea", "Three Konjac Three "Botanical Gardens and Taxonomy Research Projects" including the Research of Plant Molecular Pedigree Geography "passed the acceptance on January 15.
According to the management methods of the Knowledge Innovation Project of the Academy of Sciences, the Division of Integrated Biology invited Researcher Ding Wenjun, Dean of the School of Life Sciences of the Graduate School of the Chinese Academy of Sciences, Researcher Wu Guojiang of the South China Botanical Garden of the Chinese Academy of Sciences, Researcher Yang Junxing of the Kunming Institute of Zoology, the Chinese Academy of Sciences, and Chen Weiping, Director of the Asian and African Division of the International Cooperation Bureau of the Chinese Academy of Sciences Researcher Cao Min, deputy director of Banna Garden and other experts formed an acceptance expert group. The expert group listened to the work summary reports of the three projects and reviewed the relevant materials. After questioning and defending, they agreed that the three projects undertaken by the park had completed the research content and main assessment indicators of the mission statement and agreed to pass the project. acceptance.
The batch of projects includes plant system classification and conservation research. The completion is good and the main results are:
1. "Conservation, development and utilization of South American oil vine (Plukenetia volubilis Linneo)": (1) This project systematically studied the phenological rhythm changes of South American oil vine in Xishuangbanna area; mastered the rapid propagation of South American oil vine (cutting, seeding) And tissue culture breeding) and key techniques for high-yield cultivation, to establish a high-yield demonstration cultivation base of 100 acres, with an average yield of 150 kg of dry seeds per mu; and a preliminary discussion on the response mechanism of South American oil vine yield and quality with different altitude regions. (2) Published 5 papers, including 2 SCI papers; applied for 6 patents and authorized 4; obtained the certificate of "Forest Seed Certificate" certified by the Yunnan Forest Variety Examination Committee; 1 scientific and technological achievement was identified.
2. "Physical development and pedigree biogeographic study of the endemic plant of the ivory genus (Roscoea) in Himalayan": (1) The project collected 3588 individuals of 17 species or varieties of ivory genus (65.4% of the genus). The nuclear gene ITS and the chloroplast genes psbA and trnL-F were used as molecular sequence markers to find that the ancient haplotypes of the ivory genus are distributed in the Himalaya region, while the modern haplotype diversity center is in the Hengduan Mountains area. The haplotypes do not overlap, and the differentiation time is at least 15-17 million years ago. The analysis of the niche model shows that the dividing line between the east and the west is located in the north-south trending section of the Yarlung Zangbo River, which forms a non-overlapping distribution pattern of ivory ginseng species and haplotypes in the east and west. (2) A hybrid group of large flower ivory ginseng and early flower ivory ginseng was found in natural populations, and the existence of this natural hybridization phenomenon was confirmed through molecular detection. Based on the flowering phenology records of the ivory genus wax leaf specimens, combined with the observation of the actual phenology, the flowering phenology of the three ivory genus plants distributed in the central Himalayan was delayed by 15-30 days compared with 100 years ago. This phenomenon may be Alpine plants have a physiological response to global warming.
3. "Molecular pedigree geography study of three konjac plants": (1) The project is a sequence analysis of three fragments of chloroplasts from 322 samples of 25 populations of tiger beard Geographical isolation provides molecular evidence, as well as credible data support for paleoflora, paleogeography, and paleoclimate changes; by using nuclear fragments ITS and chloroplast fragments, 36 samples of 6 species of the genus PCR amplification and sequencing to verify and recommend the rbcL + matK fragment as the barcode for the species identification, and ITS as the candidate barcode. (2) Published 2 articles in domestic core journals and 1 SCI paper.
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